Rapid genotyping of Toxoplasma gondii by pyrosequencing
نویسندگان
چکیده
منابع مشابه
Genotyping and detection of multiple infections of Toxoplasma gondii using Pyrosequencing.
A Pyrosequencing assay, based on SAG2 gene polymorphisms, was designed for genotyping and detection of multiple infections of Toxoplasma gondii. The assay was tested on samples spiked with DNA from single and multiple genotypes of T. gondii and also on a DNA sample from the brain of a rat with multiple infections. To evaluate the comparative efficacy of the assay, identical samples were also an...
متن کاملGenotyping of Toxoplasma gondii isolates from chickens from India.
The present study was undertaken to isolate and genotype Toxoplasma gondii from free-range chickens (Gallus domesticus) from villages in Maharashtra and Tamil Nadu states of central and south India, respectively. Blood, heart, and brain from a total of 741 chickens were examined for T. gondii infection. Antibodies to T. gondii, as assayed with the modified agglutination test (MAT >or = 1:5) wer...
متن کاملMolecular Detection and Genotyping of Toxoplasma gondii from Clinical Samples
Over the past two decades, molecular diagnosis of toxoplasmosis, which is based on the detection of T. gondii DNA in clinical samples, became an indispensable laboratory test. This method is independent of the immune response, and depending on methodological approach, may facilitate more accurate diagnosis, especially in cases in which inadequacy of conventional methods is faced with deteriorat...
متن کاملIsolation and Genotyping of Toxoplasma gondii in Brazilian Dogs
Strains of Toxoplasma gondii in Brazil are highly genetically diverse compared to strains from North America and Europe. Dogs are epidemiologically important because they act as sentinels for T. gondii infections in humans and are good indicators of environmental contamination. The aim of this study was to isolate and genetically characterize T. gondii strains from tissues of naturally infected...
متن کاملGenotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis.
Although the Korean isolate KI-1 of Toxoplasma gondii has been considered to be a virulent type I lineage because of its virulent clinical manifestations, its genotype is unclear. In the present study, genotyping of the KI-1 was performed by multilocus PCR-RFLP and microsatellite sequencing. For 9 genetic markers (c22-8, c29-2, L358, PK1, SAG2, SAG3, GRA6, BTUB, and Apico), the KI-1 and RH stra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2007
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2006.01661.x